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Potomac Affinity Proteins
Step 3: Fluorescent Signaling
The addition of a cleavable signaling peptide creates a highly amplified signal.
Guide to
Symbols
Initiating Protease
Self Amplifying Complex
Self Amplifying Protease
Signaling Peptide
The key to detection is the addition of a signaling peptide. Intact, it does not provide any fluorescent signal. However, it can be cleaved by free protease which activates the fluorescence.
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