Step 3: Fluorescent Signaling

The addition of a cleavable signaling peptide creates a highly amplified signal.

Guide to

Symbols

Initiating Protease

initiator.jpg

Self Amplifying Complex

Copy of blue complex new.jpg

Self Amplifying Protease

Copy of blue active.jpg

Signaling Peptide

Copy of peptide_v2.jpg

The key to detection is the addition of a signaling peptide. Intact, it does not provide any fluorescent signal. However, it can be cleaved by free protease which activates the fluorescence.