Step 3: Fluorescent Signaling

The addition of a cleavable signaling peptide creates a highly amplified signal.

Guide to


Initiating Protease

Self Amplifying Complex

Self Amplifying Protease

Signaling Peptide

The key to detection is the addition of a signaling peptide. Intact, it does not provide any fluorescent signal. However, it can be cleaved by free protease which activates the fluorescence. 

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