Step 1: Sensitive Detection
Detection of an initiator protease is key to the Pro-CR Technology
Self Amplifying Complex
Self Amplifying Protease
The above slideshow depicts how an initiator protease causes the activation of our self-amplifying protease. The red initiator cuts the cleavage sequence of the inhibitor, activating the blue protease.
EXAMPLE: Linkage to binding molecules
In the ProCR version of an ELISA (enzyme-linked immunosorbent assay), the target protein is immobilized on a solid support, an antibody conjugated to an initiating protease forms a complex with the target protein, and then self-amplifying complex and substrate are added to amplify the signal from the conjugated protease and convert its concentration into a time signature.